HFE 3 Mutation Panel, HFE MSQ 1.1

Kras-1, 2, 3 Mutation qPCR Panel, Kras qPCR 1.1

It is suitable for in vitro diagnostic use. Intended for professional use only.

The Kras-1, 2, 3 Mutation qPCR Panel Kit is designed for the rapid and reliable detection of key mutations in the KRAS gene, including exon 2 (codons 12, 13), exon 3 (codons 59, 61), and exon 4 (codons 117, 146). This kit ensures high sensitivity and specificity for the identification of gene mutations.

FEATURES

Comprehensive mutation detection with 8 different primer-probe mixtures.
Amplification of endogenous control gene.
Assurance with positive control DNA for all mutations.
Real-time amplification with sequence-specific probe sets labeled with FAM, HEX, and Cy5.
Detection of mutations at low percentages.
Compatibility with DNA extracted from fresh, frozen, or FFPE tissue.

SAMPLE PREPARATION

DATA INTERPRETATION

Figure 1: Analysis graph of KRAS G12A for positive and negative samples using Kras-1,2,3 Mutation qPCR Panel, Kras qPCR 1.1 kit.

ORDER INFORMATION

CATALOG NO	01008025: Kras-1,2,3 Mutation qPCR Panel, Kras qPCR 1.1 (25 Tests)
	01008050: Kras-1,2,3 Mutation qPCR Panel, Kras qPCR 1.1 (50 Tests)
	01008100: Kras-1,2,3 Mutation qPCR Panel, Kras qPCR 1.1 (100 Tests)
SAMPLE TYPE	FFPE Tissue and Plasma
TEST CONTENT	K-RAS Primer Mixes, PCR Master Mixes, DNA (approximately 10-100 ng)
TARGET GENE and LOCI	Codon 12 (exon 2): G12C (c.34G>T), G12R (c.34G>C), G12S (c.34G>A), G12A (c.35G>C), G12D (c.35G>A), G12V (c.35G>T) Codon 13 (exon 2): G13D (c.38G>A) Codon 59 (exon 3): A59G (c.176C>G), A59T (c.175G>A), A59E (c.176C>A) Codon 61 (exon 3): Q61K (c.181C>A; c.180_181delinsAA), Q61L (c.182A>T), Q61R (c.182A>G), Q61H (c.183A>C; c.183A>T) Codon 117 (exon 4): K117N (c.351A>C; c.351A>T) Codon 146 (exon 4): A146P (c.436G>C), A146T (c.436G>A), A146V (c.437C>T)
STORAGE CONDITIONS and SHELF LIFE	Should be stored at -20°C and in the dark. Shelf life is 12 months.
COMPATIBLE DEVICES	Compatible with all 4-channel qPCR devices.

Note: Product specifications and information may vary, please refer to the most current information and protocols from the product's user manual or the manufacturer.