NPM1, Fragment Analysis Panel, NPM1 FRG 1.1

FLT3 Mutation Detection Panel, FLT3 MSQ FRG 1.1

For in vitro diagnostic use. Intended for professional use only.

FLT3 Mutation Detection Panel, FLT3 MSQ FRG 1.1, FLT3 (fms-related tyrosine kinase 3) gene, is the most commonly mutated gene in acute myeloid leukemia (AML) and is associated with the pathogenesis of the disease.

Two significant types of FLT3 gene mutations have been identified in acute myeloid leukemias. These are FLT3 Internal Tandem Duplication (FLT3-ITD) and FLT3 Tyrosine Kinase Domain (FLT3-TKD) mutations, both leading to the activation of FLT3. The most common mutation type in AML patients is FLT3-ITD mutation, observed in 20-30% of the patient population. FLT3-D835 mutation occurs in approximately 7% of AML cases and is associated with a poor prognosis. The rapid, accurate, and specific detection of FLT3 gene mutations allows for the determination of the drug substance and dosage to be used in the treatment of the disease.

FEATURES

  • Single Tube Multiplex: The PCR process conducted in a single tube enables the multiplex detection of FLT3 gene ITD and TKD mutations.
  • Simple and Fast: Working in a single tube multiplex format allows for quick restriction enzyme cleavage and capillary electrophoresis, ensuring straightforward and rapid results.
  • High Sensitivity: The FLT3 Gene Mutation Detection Panel can identify even low-level mutations due to its high sensitivity. This facilitates accurate and comprehensive genetic analysis.

DATA INTERPRETATION

Figure 1: The FLT3 Mutation Detection Panel, displayed on the ABI 3500 Genetic Analyzer Device using the FLT3 FRG 1.1 Kit

ORDER INFORMATION

CATALOG NO

MSFRHLKSFLT01-25: The FLT3 Mutation Detection Panel, FLT3 FRG 1.1 (25 tests)

 

MSFRHLKSFLT01-50: The FLT3 Mutation Detection Panel, FLT3 FRG 1.1 (50 Test)

 

MSFRHLKSFLT01-100: The FLT3 Mutation Detection Panel, FLT3 FRG 1.1 (100 Test)

SAMPLE TYPE

Whole Blood with EDTA Tube, Bone Marrow.

TEST CONTENT

 FLT3 ITD-D835/D836 PCR Master Mix, Restriction Enzyme, RE Buffer, ddH2O

TARGET GENE and LOCI

FLT3 Gene Internal Tandem Duplication (ITD) region, FLT3 Gene D835/D836 mutations

STORAGE CONDITIONS AND SHELF LIFE

Should be stored and – 20 °C in the dark. Shelf life is 12 months.

COMPETIBLE DEVICES

All PCR devices, ABI 3130, 3500 and other